4096 Some Assembly Required by Mr E

This was another imaginative and superbly crafted puzzle from Mr. E. Most clues had ‘arbitrary irregularities’ which the solver had to recognise and use. Most clues had an extra word which replaced one letter in the wordplay. All the letters of the alphabet were used. Replacing the letters of the answers to normal clues by their corresponding letters gave three new clues. These were ONUS = “Each expert’s holding prize” for APIECE (1dn); ZARF = “Grandma’s tiramisu uses wine” for ASTI (30ac); HIVE = “Mistakenly cite Hopi tongue” for ETHIOPIC (5ac). The remaining letters produced two further unclued entries, which generated clues for the final two unclued entries: PJCKMQW = “African city grew up by dry area” for ROSETTA (25ac); LXDYBTG = “Our people started rioting after hurtful publications”, for ILLUSTRATEDS (7dn).

Extra words and missing letters are shown in [ ]
 
Across
1 Australian [city] last to regulate drug? Not true in Perth (4)
AJEE A (Australian) + [J] + (regulat)E + E
12 Railroad [expert's] concerned with oxygen - it affects the weather (6, two words)
EL NIÑO EL + [N] + IN + O
13 Deviously used, barrels of [wine] and bull will get you sincere statements
(12, two words)
DOUBLE BLUFFS Anagram USED + B + OF + BULL + [F]
14 Old slave's [tongue] nearly cut (4)
ESNE [E] + SNE(d)
18 [Each] lecturer held in care of the Queen's prison (6)
COOLER [O] + L (lecturer) in CO + ER
19 Research in progress about [holding] would be widely known (7)
RESOUND RES + [U] in ON + ‘D
21 Set free [after] terrible stir in American court (8)
ABSTRICT A (American) + [B] + Anagram STIR + CT
22 Somewhere in Spain, to the west, a volcano [grew] - yes, indeed! (8)
ALICANTE ETNA + [C] +I + LA reversed
27 What could make Queen Anne cabinet reverse [hurtful] old fine? (6)
WALNUT [T] + UNLAW reversed
31 Soldiers eating [tiramisu] after it's clumsily frosted - great occasion (12, two words)
ARMISTICE DAY  Anagram IT’S + ICED in ARMY containing [A]
32 Stewards make their way through [prize] birds (6)
REEVES 2 definitions  REEVE = make their way through + [S]
34 [Uses] among foster staff for one who was possessed (8)
FREEDMAN [R] in FEED + MAN
35 A vote for accommodating [people] from the East is tempting (4)
SEXY [X] in YES reversed
 
Down
2 Cut waste [by] getting into green racket? (9)
ECONOMISE  [M] in ECO + NOISE
3 European Union holding [up] half of team's money (6)
EKUELE [K] in EU + ELE(ven)
4 [Started] after rough Belém teams I represented (12)
EMBLEMATISED Anagram BELEM TEAMS + [D]
6 Ancient peer's [area] in support, right? (5)
TWEER [W] in TEE + R
8 An obligation that we are under (4)
ONUS obligation is ON US
9 [Cite] new fantastic early stage (7)
INFANCY [I] + N + FANCY
10 [Mistakenly], socialist lifts weapon (4)
COSH H + SOC (reversed)
15 A thousand find fault with [Grandma's] stony face (6)
KRANTZ K + RANT + [Z]
16 Money is found by partners in African river (6)
PESEWA ‘S (is) + EW IN [P] + EA
17 [Dry] Beaune is bad? Who knows? (9, two words)
QUIÉN SABE [Q] + Anagram BEAUNE IS
20 Retort for [our] ore needs repair (7)
FLOORER Anagram FOR [L] ORE
23 Perhaps Knight [Publications] first to speak about girlfriend (6)
GLADYS LADY in [G] + S(peak)
24 Mean to incite [rioting] (6)
STINGY STING + [Y]
26 [Hopi] in trouble with a city in Spain (5)
AVILA [V] in AIL + A
28 Cup holder South Africa upset France (4)
ZARF ZA + FR reversed
29 At end of month I have to move into a box (4)
HIVE (mont)H + I’VE